Bovine IgG (H+L chain) Goat Polyclonal Antibody

CAT#: R1616HRP

Bovine IgG (H+L chain) goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting (1/5,000-1/100,000, Western or dot blot), ELISA (1/10,000-1/200,000), Immunoperoxidase Electron Microscopy and Immunohistochemistry (1/500-1/5,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
• Reactivities: Bovine
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Bovine IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Bovine and Bovine Serum.
• Formulation: 0.01M Potassium Phosphate, 0.25M Sodium Chloride, pH 7.2 containing 15 mg/ml BSA (IgG and Protease free) as stabilizer.
  Label: HRP
  State: Lyophilized purified IgG fraction.
  Preservative: None
  Label: Horseradish Peroxidase
• Reconstitution Method: 2 ml of deionized water or equivalent is suggested for reconstitution, the product has been overfilled to ensure complete recovery.
• Concentration: 0.8 mg/ml (at time of lyophilization of lyophilization)
• Purification: Immunoaffinity Chromatography.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration.
  Restore with 2.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.