Bovine IgG (H+L chain) Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | FC, IHC |
Recommended Dilution | Suitable for Immunomicroscopy and Fow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
Reactivities | Bovine |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Bovine IgG whole molecule. |
Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Bovine IgG and Bovine Serum. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 contaning 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml Polyethylene Glycol (PEG-8000) as stabilizer. Label: Texas Red State: Lyophilized purified IgG fraction. Label: - Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption emission: 596 nm / 620 nm Molar radio: 5.0 moles Texas Red per mole of Goat IgG |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
Concentration | 2.0 mg/ml (before lyophil. by UV absorbance at 280 nm) |
Purification | Immunoaffinity chromatography. |
Conjugation | Texas Red |
Storage | Store vial at 2-8°C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. |
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