Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody

CAT#: R1617HRP

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
  Recommended Dilutions:
  ELISA: 1/10,000-1/150,000.
  Western blot: 1/1,000-1/5,000.
  Immunohistochemistry: 1/1,000-1/10,000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Chicken IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Chicken IgG and Chicken Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
  Label: HRP
  State: Lyophilized purified Ig fraction.
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (before lyophil. by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration.
  Restore with 1.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.