Rat IgG2c (heavy chain) Mouse Monoclonal Antibody [Clone ID: MARG2c-5]

CAT#: SM1034P

Rat IgG2c (heavy chain) mouse monoclonal antibody, clone MARG2c-5, Purified

Specifications

Product Data

• Clone Name: MARG2c-5
• Applications: ELISA
• Recommended Dilution: ELISA: 5 µg/ml as Coating antibody.
  This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified Rat IgG2c (Cat.No SA024).
  Flow Cytometry: Use 10 µl of 1/200-1/1000 diluted antibody to label 10⁶ cells in 100 µl.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: IgG2c (Rat strain LOU/c). Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
• Specificity: This antibody reacts with Gamma 2c heavy chain (determined by immunodot).
  The specificity of monoclonal antibodies against IgG subclasses is determined in a range of optimal concentrations. Increasing the concentration of the first or second antibody can induce cross-reactions with other subclasses.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.