IgG (Immunglobulin G, light chain), mouse anti porcine, clone PHE425

CAT#: TA363875

IgG (Immunglobulin G, light chain), mouse anti porcine, clone PHE425

Product Images

Performance of monoclonal antibody PHE425 in ELISA

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  TA363875, Clone PHE425, swine spleen, paraffin section

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  TA363875, Clone PHE425, swine pancreas, paraffin section

Specifications

Product Data

• Clone Name: PHE425
• Applications: ELISA, IHC, WB
• Recommended Dilution: Tested for immunohistochemistry (IHC), ELISA and Western Blot, other applications not yet tested. Approximate working dilutions: IHC, frozen sections: 0.1µg/ml (1:2000) IHC, paraffin sections: 0.25µg/ml (1:800); Proteinase K pretreatment for antigen retrieval is recommended. Western Blot: 0.05 – 0.5µg/ml. ELISA: 20 - 50ng/ml (EC50 of 1ng/ml illustrated below) Optimal dilutions should be determined by the end user.
• Reactivities: Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Porcine liver extract.
• Specificity: This clone PHE425 reacts specifically with porcine IgG, but not with human or rabbit IgG. Western Blot analysis with purified porcine IgG under non-reducing and reducing conditions yielded bands at 150kDa and 25kDa, respectively, consistent with intact IgG and kappa or lambda light chain, respectively.
  
  Epitope: The antigen has been identified as porcine immunoglobulin light chain, the subclass has not been further identified.
  
  Distribution: Tissue sections: The antibody reacts with the light chains of immunoglobulin G in porcine organs, predominantly with immunoglobulins in the interstitial fluid (ISF).
• Formulation: Affinity purified from cell culture supernatant, lyophilized. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.2mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA) as a stabilizer and 0.05% (v/v) Kathon CG as a preservative.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: 1 year at 4° - 8°C. Avoid repeated thawing and freezing of the reconstituted antibody.