mCherry Goat Polyclonal Antibody

CAT#: AB0081-200

Goat Polyclonal Anti-mCherry Antibody

Product Images

Anti-mCherry Ab at 1/1,000 dilution; 293HEK cells transduced with myc-mCherry Ad; lysates at 100 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution

• 

  Immunofluorescence – anti-mCherry Ab (AB0081) in 293HEK cells transfected with mCherry-Rab1a at 1/50 dilution; cells were fixed with 4% of PFA;

• 

  Immunogold labeling of RPE, in vivo injected with mCherry expressing vector;

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB 1:500-1:5,000; IF 1:50-1:500; IHC (paraffin) 1:50-1:500; IHC (frozen) 1:50-1:500
• Target: Red Fluorescent, tdTomato, mCherry
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Purified recombinant peptide produced in E. coli.
• Isotype: IgG
• Formulation: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
• Concentration: 3mg/ml
• Purification: epitope affinity purified
• Conjugation: Unconjugated
• Background: Red Fluorescent Protein
• Note: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody (AB0081) recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).