V5

CAT#: AB0096-500

Goat Polyclonal Anti-V5 Antibody

Product Images

Anti-V5 Ab at 1/1,000 dilution; MBP-V5 recombinant protein and 293 cells transfected with V5-mCherry; lysate at 100 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

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  Myo5a deficient melanocytes transiently transduced with adenovirus expressing V5-tagged Myo5a, fixed and stained with anti-V5 Ab (1:1,000); donkey anti-goat Alexa 568 labelled secondary Ab was used;

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB 1:500-1:5,000; IF 1:500-1:2,000
• Target: V5-tagged proteins
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Purified recombinant peptide produced in E. coli.(MGKPIPNPLLGLDSTNPMGKPIPNPLLGLDSTNPMGKPIPNPLLGLDSTNPMGKPIPNPLLGLDSTNPMGKPIPNPLLGLDSTNPGRLERPHRD)
• Isotype: IgG
• Formulation: Polyclonal antibody supplied as a 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
• Concentration: 3mg/ml
• Purification: epitope affinity purified
• Conjugation: Unconjugated
• Background: P and V proteins of the Paramyxovirus of simian virus SV5
• Note: Reacts specifically with V5-tagged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.