tdTomato Goat Polyclonal Antibody

CAT#: AB8181-200

Goat Polyclonal Anti-tdTomato Antibody

Product Images

Anti-tdTomato Ab at 1/1,000 dilution; 293HEK cells transfected with cDNA; lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

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  Immunofluorescence – anti-tdTomato Ab in 293HEK cells transfected with cDNA at 1/50 dilution; cells were fixed with 4% of PFA;

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB 1:500-1:2,000; IF 1:50-1:500; IHC (paraffin) 1:50-1:500; IHC (frozen) 1:50-1:500
• Target: tdTomato, mCherry, red fluorescent
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Purified recombinant peptide produced in E. coli.
• Isotype: IgG
• Formulation: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
• Concentration: 3mg/ml
• Purification: epitope affinity purified
• Conjugation: Unconjugated
• Background: Red Fluorescent Protein
• Note: In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody (AB8181) is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).