Carrier-free (BSA/glycerol-free) Mouse monoclonal mRaspberry/AmCyan1 antibody, clone OTI2A6

CAT#: CF180029

Carrier-free (BSA/glycerol-free) Mouse monoclonal mRaspberry/AmCyan1 antibody, clone OTI2A6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mRaspberry (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mRaspberry/AmCyan1 (TA180029, 1:2000).

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AmCyan1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mRaspberry/AmCyan1 (TA180029, 1:2000).

Specifications

Product Data

• Clone Name: OTI2A6 (formerly 2A6)
• Applications: WB
• Recommended Dilution: WB: 1:2000
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: This antibody is raised against a recombinant protein of mRaspberry expressed in HEK293 cells. It recognizes both mRaspberry and AmCyan1 proteins.
• Isotype: IgG1
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Predicted Protein Size: 25.5 kDa