Carrier-free (BSA/glycerol-free) Mouse monoclonal mGFP/mCFP/mYFP/KillerRed antibody, clone OTI2F6

CAT#: CF180076

Carrier-free (BSA/glycerol-free) Mouse monoclonal mGFP/mCFP/mYFP/KillerRed antibody, clone OTI2F6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mGFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mGFP/mCFP/mYFP/KillerRed (TA180076, 1:500).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mCFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mGFP/mCFP/mYFP/KillerRed (TA180076, 1:500).

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mYFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mGFP/mCFP/mYFP/KillerRed (TA180076, 1:500).

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KillerRed (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mGFP/mCFP/mYFP/KillerRed (TA180076, 1:500).

Specifications

Product Data

• Clone Name: OTI2F6 (formerly 2F6)
• Applications: WB
• Recommended Dilution: WB: 1:500
• Target: mGFP, mCFP, mYFP, KillerRed
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: This antibody is raised against a recombinant protein of mGFP expressed in HEK293 cells. It recognizes mGFP, mCFP, mYFP and KillerRed proteins.
• Isotype: IgG1
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: Stable for 1 year at -20°C from date of shipment.
• Predicted Protein Size: 27.3 kDa