Carrier-free (BSA/glycerol-free) HA mouse monoclonal antibody, clone CB051

CAT#: CF180128

Carrier-free (BSA/glycerol-free) HA mouse monoclonal antibody, clone CB051

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or HA tagged LGALS3 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HA.

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  Western Blot comparison of anti-HA Tag antibodies from OriGene (TA180128), BioLegend (mms-101p), and Abcam (ab18181). 0.04ul (Lane 1, 3, 5) and 0.2ul (Lane 2, 4, 6) of HA-PARD6B lysate (recombinant proteins with HA tag at the N-terminus), blotted with 1:1000 dilution of TA180128 (lane 1-2), mms-101p (Lane 3-4), and ab18181 (Lane 5-6).

Specifications

Product Data

• Clone Name: CB051
• Applications: WB
• Recommended Dilution: WB 1:2000
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Monoclonal antibody is produced by immunizing mice with a synthetic peptide from influenza hemaglutinin (YPYDVPDYA) coupled to KLH
• Isotype: IgG1
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Synonyms: ANTI-HA; Anti-HA Antibody; Anti HA; Anti HA Antibody; ANTI INFLUENZA