Anti-Myc and Anti-DDK monoclonal antibodies

CAT#: TA150014

Anti-Myc and Anti-DDK monoclonal antibodies

Product Images

Immunoblot analysis of randomly picked 7 different Myc/DDK tagged overexpression lysates and Myc/DDK tagged Western molecular weight marker (MWM1001) with TA50011 at 1:2,000.

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  Immunoblot analysis of four different Myc-DDK tagged over-expression lysates and one Empty vector transfected 293T cells with TA150121 at 1:500.

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  Hela cells transiently transfected with Myc/DDK tagged GFAP ORF cDNA clone were immunostained with 4C5 anti-DDK antibody (TA50011, 1:1000), and then colored in green with Alexa 488 conjugated secondary antibody (1:2000). The nuclei (blue) were counterstained with DAPI.

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  Immunofluorescent staining of COS7 cells transiently transfected with Myc-DDK tagged MYD88 (Cat# RC202253).

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  Immunofluorescent staining of COS7 cells transiently transfected with Myc-DDK tagged RASSF1 (Cat# RC213525).

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  Immunofluorescent staining of COS7 cells transiently transfected with Myc-DDK tagged MEF2C (Cat# RC220584).

• Protein purification of a human protein from over-expression lysate with anti-DDK antibody affinity column (TA50011, clone 4C5)

• Immunoprecipitation (IP) of Myc-DDK tagged HMGA2 and FCGR2A over expression lysate by anti-Myc mouse monoclonal antibody (TA150121) or with goat anti-mouse conjugated magnetic beads directly.

• HEK293T cells transfected with either Myc/DDK tagged PROM2 ORF overexpression plasmid (red) or empty vector control plasmid (blue) were immunostained with OriGene anti-DDK antibody (TA50011, 1:1000) and analyzed by flow cytometry.

• HEK293T cells transfected with either Myc-DDK tagged PA2G4 plasmid (Red) or empty vector plasmid (Blue) were immunostained with anti-Myc mouse monoclonal antibody (TA150121) at 1:100, and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: 9E10/4C5
• Applications: FC, IF, IP, WB
• Recommended Dilution: FC: 1:100-1:1000, WB: 1:1000, IP: 1:200, IF: 1:200
• Target: Myc and DDK (FLAG) tags
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Anti-Myc: AEEQKLISEEDLLRKRREQLKHKLE conjugated to KLH, corresponding to C terminal amino acids 408-432 of human c-Myc; Anti-DDK: a synthetic peptide (DYKDDDDK) coupled to KLH.
• Isotype: IgG1/IgG2a
• Formulation: PBS (pH7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Synonyms: ANTI-DDK AND MYC; ANTI-MYC-DDK; ANTI-MYC/DDK; ANTI-MYC AND ANTI-DDK; ANTI-MYC ANTI-DDK; ANTI-MYC DDK; ANTI-MYC MAB; ANTI-MYC MONOCLONAL ANTIBODY; ANTI-MYC TAG; ANTI CMYC; ANTIMYC; ANTI MYC/DDK; ANTI MYC DDK; ANTI MYC TAG