Rabbit polyclonal eGFP antibody

CAT#: TA150032

Rabbit polyclonal eGFP antibody

Product Images

Western blot analysis of GFP expression in transfected 293T cell line by GFP polyclonal antibody (12% SDS-PAGE, TA150032 diluted at 1:10000). A: Non-transfected lysate; B: GFP transfected lysate.

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  Western blot analysis of 1 ug of whole cell lysate with anti-GFP antibody TA150032 diluted at 1:10000. A: Non-transfected 293T lysates; B: GFP transfected 293T lysates; C: RFP transfected 293T lysates.

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  Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( TA150032, 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.

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  GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody (TA150032, 1:15000).

Specifications

Product Data

• Applications: IF, IP, WB
• Recommended Dilution: IF, IP, WB
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant full length protein of eGFP (1-265aa)
• Isotype: IgG
• Formulation: 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative
• Concentration: 1 mg/ml
• Storage: Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
• Predicted Protein Size: 29.0 kDa