Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic Beads

CAT#: TA150039S

Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic Beads

Product Images

Immunoprecipitation (IP) was performed with overexpression lysate expressed with tGFP expression vector (Cat# PS100010) or control lysate transfected with pCMV-Entry empty vector (Cat# PS100001). Briefly, 20uL anti-tGFP (2H8) magnetic beads (Cat# TA150039) were incubated with tGFP overexpression lysate or negative control lysate overnight at 4°C, respectively. After washing three times with RIPA buffer, 20uL 4x SDS sample buffer was applied to the lysate-beads mixture. The samples were boiled at 95°C for 10min. The IP sample supernatants were fractionated on SDS-PAGE gel, blotted onto nitrocellulose membrane. The IP efficiency was evaluated by using HRP-conjugated anti-tGFP antibody (Cat# TA150043). Lane 1: anti-tGFP (2H8) magnetic beads (20uL) with tGFP expression lysate; Lane 2: anti-tGFP magnetic beads (20uL) with negative control lysate.

Specifications

Product Data

• Clone Name: OTI2H8 (formerly 2H8)
• Applications: IP, Protein Purification
• Recommended Dilution: IP, Protein Purification
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Anti-tGFP monoclonal antibody was produced by immunizing mice with purified tGFP protein expressed in HEK293T cell.
• Isotype: IgG2b
• Formulation: PBS (pH7.4) containing 0.1% BSA, 0.02% NaN3 and 50% glycerol with final concentration 5mg/mL beads
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Predicted Protein Size: 26 kDa