Human IL-17 ELISA Kit (48-well)
USD 290.00
3 Weeks*
Specifications
Product Data | |
Format | 48-well strip plate |
Assay Type | Solid Phase Sandwich ELISA |
Assay Length | 3 hours |
Signal | Colorimetric |
Curve Range | 15.625-1000pg/ml |
Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
Sample Volume | 20 uL |
Specificity | Natural and recombinant Human IL-17 Ligand |
Sensitivity | 7pg/mL |
Reactivities | Human |
Interference | No significant interference observed with available related molecules. |
Components |
|
Background | IL-17, originally identified as mouse cytotoxic T lymphocyte-associated antigen-8 (CTLA-8) , is produced by activated T lymphocytes, primarily by memory T cells . IL-17 appears to mediate communication between the immune system and the hematopoietic system. IL-17 is a disulfide-linked homodimer . Each polypeptide has 155 amino acid (aa) residues (predicted mass = 17.5 kDa), including a 19 aa residue hydrophobic leader sequence . There are six cysteines plus one potential N-linked glycosylation site, which is variably glycosylated, at least with recombinant proteins . The aa sequence of human IL-17 is 63% and 58% identical to mouse and rat IL-17 and 72% identical to the thirteenth ORF of Herpesvirus saimiri . There is at least some species specificity for in vitro action on bone-marrow stromal cells . IL-17 mediation of T cell communication with the hematopoietic system is suggested by two observations. T cell-derived IL-17 induces fibroblasts to produce IL-6, IL-8, ICAM-1 and G-CSF, apparently by an NF-.B-mediated mechanism . IL-6 in turn promotes development of granulocyte/macrophage colonies, and G-CSF directs development of neutrophils . IL-17 also enhances proliferation of partially activated T cells and upregulates nitric oxide (NO) production in osteoarthritic cartilage . |
Gene Symbol | IL17A |
Standard Curve |
Representative standard curve for IL-17 ELISA. IL-17 was diluted in serial two-fold steps in Sample Diluent.
|
Documents
{0} Product Review(s)
Be the first one to submit a review