Human IL-17 ELISA Kit (96-well)

CAT#: EA100074

Human IL-17 ELISA Kit (96-well)

Product Images

Representative standard curve for IL-17 ELISA. IL-17 was diluted in serial two-fold steps in Sample Diluent.

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 15.625-1000pg/ml
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Human IL-17 Ligand
• Sensitivity: 7pg/mL
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with antibody to human IL-17 (8x12)
  • 1 vials human IL-17 Standard lyophilized,1000 pg/ml upon reconstitution
  • 1 vials concentrated Biotin-Conjugate anti-human IL-17 antibody
  • 1 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 2 pieces Adhesive Films
  • package insert
• Background:

  IL-17, originally identified as mouse cytotoxic T lymphocyte-associated antigen-8 (CTLA-8) , is produced by activated T lymphocytes, primarily by memory T cells . IL-17 appears to mediate communication between the immune system and the hematopoietic system.

  IL-17 is a disulfide-linked homodimer . Each polypeptide has 155 amino acid (aa) residues (predicted mass = 17.5 kDa), including a 19 aa residue hydrophobic leader sequence . There are six cysteines plus one potential N-linked glycosylation site, which is variably glycosylated, at least with recombinant proteins . The aa sequence of human IL-17 is 63% and 58% identical to mouse and rat IL-17 and 72% identical to the thirteenth ORF of Herpesvirus saimiri . There is at least some species specificity for in vitro action on bone-marrow stromal cells .

  IL-17 mediation of T cell communication with the hematopoietic system is suggested by two observations. T cell-derived IL-17 induces fibroblasts to produce IL-6, IL-8, ICAM-1 and G-CSF, apparently by an NF-.B-mediated mechanism . IL-6 in turn promotes development of granulocyte/macrophage colonies, and G-CSF directs development of neutrophils . IL-17 also enhances proliferation of partially activated T cells and upregulates nitric oxide (NO) production in osteoarthritic cartilage .

• Gene Symbol: IL17A
• Standard Curve:
  
  Representative standard curve for IL-17 ELISA. IL-17 was diluted in serial two-fold steps in Sample Diluent.