Rat IL-2 ELISA Kit (48-well)

CAT#: EA100159

Rat IL-2 ELISA Kit (48-well)

Size: 1 x 48 wells 1 x 96 wells


USD 290.00

3 Weeks*

Size
    • 1 x 48 wells

Product Images

Specifications

Product Data
Format 48-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hour
Signal Colorimetric
Curve Range 0.03 - 2.0 ng/mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume 20 uL
Specificity Natural and recombinant Rat IL-2 Ligand
Sensitivity 7pg/mL
Reactivities Rat
Interference No significant interference observed with available related molecules.
Components
  • Aluminium pouches with a Microwell Plate coated with monoclonal antibody to rat IL-2 (8 6)
  • 2 vials rat IL-2 Standard lyophilized,4000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-rat IL-2 antibody
  • 2 vials Streptavidin-HRP solution,
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert
Background

Interleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by mitogen- or antigen-activated T lymphocytes . It plays a key role in promoting the clonal expansion of antigen-specific T cells. In addition, IL-2 has been shown to mediate multiple immune responses on a variety of cell types.

IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendocytes. IL-2 is involved in treatment of cancers such as melanoma and renal cell cancer. It plays a key role in promoting the clonal expansion of antigen-specific T cells. In addition, IL-2 has also been shown to mediate multiple immune responses on a variety of cell types.

The sequence of rat IL-2 cDNA predicts a 155 amino acid (aa) residue precursor glycoprotein containing a 20 aa residue signal peptide that is cleaved to form the mature protein . At the amino acid sequence level, mature rat IL-2 is approximately 80% identical to mouse IL-2 .

The biological effects of IL-2 are mediated by specific cell surface receptor complexes. The functional high-affinity receptor for IL-2 is composed of three distinct polypeptide chains.

IL-2 stimulates the proliferation of thymocytes; stimulates the proliferation and differentiation of activated B cells; promotes the growth, differentiation and cytocidal activity of monocytes; induces the growth of and cytokine production and cytolytic activity by natural killer cells; enhances the production of lymphocyte-activated killer (LAK) cells; and induces the proliferation and differentiation of oligodendrocytes .

Gene Symbol Il2
Standard Curve
Representative standard curve for IL-2 ELISA. IL-2 was diluted in serial two-fold steps in Sample Diluent.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.