Rat IL-2 ELISA Kit (96-well)

CAT#: EA100160

Rat IL-2 ELISA Kit (96-well)

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hour
• Signal: Colorimetric
• Curve Range: 0.03 - 2.0 ng/mL
• Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Sample Volume: 20 uL
• Specificity: Natural and recombinant Rat IL-2 Ligand
• Sensitivity: 7pg/mL
• Reactivities: Rat
• Interference: No significant interference observed with available related molecules.
• Components:
  • Aluminium pouches with a Microwell Plate coated with monoclonal antibody to rat IL-2 (8X12)
  • 2 vials rat IL-2 Standard lyophilized,4000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-rat IL-2 antibody
  • 2 vials Streptavidin-HRP solution,
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert
• Background:

  Interleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by mitogen- or antigen-activated T lymphocytes . It plays a key role in promoting the clonal expansion of antigen-specific T cells. In addition, IL-2 has been shown to mediate multiple immune responses on a variety of cell types.

  IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendocytes. IL-2 is involved in treatment of cancers such as melanoma and renal cell cancer. It plays a key role in promoting the clonal expansion of antigen-specific T cells. In addition, IL-2 has also been shown to mediate multiple immune responses on a variety of cell types.

  The sequence of rat IL-2 cDNA predicts a 155 amino acid (aa) residue precursor glycoprotein containing a 20 aa residue signal peptide that is cleaved to form the mature protein . At the amino acid sequence level, mature rat IL-2 is approximately 80% identical to mouse IL-2 .

  The biological effects of IL-2 are mediated by specific cell surface receptor complexes. The functional high-affinity receptor for IL-2 is composed of three distinct polypeptide chains.

  IL-2 stimulates the proliferation of thymocytes; stimulates the proliferation and differentiation of activated B cells; promotes the growth, differentiation and cytocidal activity of monocytes; induces the growth of and cytokine production and cytolytic activity by natural killer cells; enhances the production of lymphocyte-activated killer (LAK) cells; and induces the proliferation and differentiation of oligodendrocytes .

• Gene Symbol: Il2
• Standard Curve:
  
  Representative standard curve for IL-2 ELISA. IL-2 was diluted in serial two-fold steps in Sample Diluent.