Human BMP-5 ELISA Kit

CAT#: EA100208

Human BMP-5 ELISA Kit

Product Images

Representative standard curve for Human BMP-5 PicoKine ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human BMP-5
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 156pg/ml-10,000g/ml
• Sample Type: Cell culture supernates, serum and Bone tissue.
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human BMP-5
• Sensitivity: <10pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human BMP-5 antibody 1
  • Lyophilized recombinant human BMP-5 standard 10ng/tubex2
  • Biotinylated anti- human BMP-5 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on human chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18KDa.
• Gene Symbol: BMP5
• Standard Curve:
  
  Representative standard curve for Human BMP-5 PicoKine ELISA Kit