Mouse IL-5 ELISA Kit

CAT#: EA100278

Mouse IL-5 ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Mouse IL-5
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 15.6pg/ml-1000pg/ml
• Sample Type: Cell culture supernates and serum.
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of mouse IL-5
• Sensitivity: <2pg/ml
• Reactivities: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- mouse IL-5 antibody 1
  • Lyophilized recombinant mouse IL-5 standard 10ng/tubex2
  • Biotinylated anti- mouse IL-5 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.
• Gene Symbol: Il5
• Standard Curve:
  
  Representative standard curve for Mouse IL-5 PicoKine ELISA Kit