Human CD95 ELISA Kit, 2 x 96-well (pre-coated)

CAT#: EA101207

Human CD95 ELISA Kit, 2 x 96-well (pre-coated)

Product Images

Representative Standard Curve

Specifications

Product Data

• Format: 2 x 96-well (pre-coated)
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 1h45min
• Signal: Colorimetric
• Curve Range: 93.75-3000 pg/ml
• Sample Type: Serum, Plasma, Cell culture supernatant
• Sample Volume: 100 µl
• Specificity: Recognizes both natural and recombinant soluble human Fas
• Sensitivity: 47 pg/ml
• Cross Reactivity: No cross reactivity with other human soluble molecules
• Interference: No significant interference observed with available related molecules.
• Components: ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
• Background: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]
• Gene Symbol: FAS
• Standard Curve:
  
  Representative Standard Curve