Human Perforin ELISA Kit, 1 x 48-well (pre-coated)

CAT#: EA101238

Human Perforin ELISA Kit, 1 x 48-well (pre-coated)

Product Images

Representative Standard Curve

Specifications

Product Data

• Format: 1 x 48-well (pre-coated)
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 2h45min
• Signal: Colorimetric
• Curve Range: 62.5-2000 pg/ml
• Sample Type: Supernatant, Buffered Solutions, Not suitable for use with serum and plasma.
• Sample Volume: 100 µl
• Specificity: Recognizes both natural and recombinant human Perforin
• Sensitivity: 40 pg/ml
• Cross Reactivity: No cross reactivity with Trail, TRAIL R3, TRAIL R4, Granzyme B, TNFa, IFN?, Fas, Fas L, IL-1b, IL-2
• Interference: No significant interference observed with available related molecules.
• Components: ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
• Background: The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
• Gene Symbol: PRF1
• Standard Curve:
  
  Representative Standard Curve