Human Perforin ELISA Kit, 1 x 48-well (pre-coated)

CAT#: EA101238

Human Perforin ELISA Kit, 1 x 48-well (pre-coated)


USD 255.00

3 Weeks*

Size
    • 1 x 48 wells (pre-coated)

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Specifications

Product Data
Format 1 x 48-well (pre-coated)
Assay Type Solid Phase Sandwich ELISA
Assay Length 2h45min
Signal Colorimetric
Curve Range 62.5-2000 pg/ml
Sample Type Supernatant, Buffered Solutions, Not suitable for use with serum and plasma.
Sample Volume 100 µl
Specificity Recognizes both natural and recombinant human Perforin
Sensitivity 40 pg/ml
Cross Reactivity No cross reactivity with Trail, TRAIL R3, TRAIL R4, Granzyme B, TNFa, IFN?, Fas, Fas L, IL-1b, IL-2
Interference No significant interference observed with available related molecules.
Components ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
Background The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Gene Symbol PRF1
Standard Curve
Representative Standard Curve

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.