IDO1 (NM_002164) Human 3' UTR Clone

CAT#: SC203025

3`UTR clone of indoleamine 23-dioxygenase 1 (IDO1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: IDO1
• Synonyms: IDO; IDO-1; INDO
• ACCN: NM_002164
• Insert Size: 647 bp
• Sequence Data:
  Insert Sequence

  >SC203025 3'UTR clone of NM_002164
  The sequence shown below is from the reference sequence of NM_002164. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGAGAAATCCCTTTTGAAGGAAGGTTAATGTAACCCAACAAGAGCACATTTTATCATAGCAGAGACATC
  TGTATGCATTCCTGTCATTACCCATTGTAACAGAGCCACAAACTAATACTATGCAATGTTTTACCAATAA
  TGCAATACAAAAGACCTCAAAATACCTGTGCATTTCTTGTAGGAAAACAACAAAAGGTAATTATGTGTAA
  TTATACTAGAAGTTTTGTAATCTGTATCTTATCATTGGAATAAAATGACATTCAATAAATAAAAATGCAT
  AAGATATATTCTGTCGGCTGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGG
  GCGGATCACAAGGTCAGGAGATCGAGACCATCTTGGCTAACACGGTGAAACCCCGTCTCTACTAAAAATA
  CAAAAAATTAGCCGGGCGCGGTGGCGGGCACCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAAT
  GGCGTGAACCTGGGAGGCGGAGCTTGCAGTGAGCCAAGATTGTGCCACTGCAATCCGGCCTGGGCTAAAG
  AGCGGGACTCCGTCTCAAAAAAAAAAAAAAAAAGATATATTCTGTCATAATAAATAAAAATGCATAAGAT
  ATAAAAAAAAAAAAAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002164.5
• Summary: 'This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]'
• Locus ID: 3620