NDUFB5 (NM_002492) Human 3' UTR Clone

CAT#: SC206604

3`UTR clone of NADH dehydrogenase (ubiquinone) 1 beta subcomplex5 16kDa (NDUFB5) nuclear gene encoding mitochondrial protein for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NDUFB5
• Synonyms: CISGDH; SGDH
• ACCN: NM_002492
• Insert Size: 466 bp
• Sequence Data:
  Insert Sequence

  >SC206604 3'UTR clone of NM_002492
  The sequence shown below is from the reference sequence of NM_002492. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCCGAAAGCAACTCCTGACAATTAAGCATTTTTTTCTCCAAATACAAAGTATATTCTCTTTATTGGAAAA
  TAAATTAATAAATATATTCTGTATTTTTGCTCTCCGTGAAAAACAAAAGAGCCTCTGACATTACTGTCTC
  TCAGTGTTGGTTCAGATTGAGGCTTTTGTTTGAGGAGTTTGGCTTCCAGTCCCCAAAGAAGGTTTAAAAT
  GTACTAATAAAAACTGGAGAAATAGGAATTTGTGAACTCCTAAAATTGTAGCAACTTTGAAAGGTTAGTG
  TTTTATTTACCTGACAAATGGAAGTTATAGAAGTCTAATAATGTTAAGCACCAGTAATTATAGTATTTTG
  TACAAATGCCTGTACTGTAGATGTCTGTATTATTGAGGGGCAACTCAGGATTTTAAGTTCCATAAAGTAT
  TGAACCAACTCTAAAATGTTAACTCCTTAATAAACAGATTCTCAGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002492.2
• Summary: 'The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]'
• Locus ID: 4711