DPAGT1 (NM_001382) Human 3' UTR Clone

CAT#: SC206778

3`UTR clone of dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DPAGT1
• Synonyms: ALG7; CDG-Ij; CDG1J; CMS13; CMSTA2; D11S366; DGPT; DPAGT; DPAGT2; G1PT; GPT; UAGT; UGAT
• ACCN: NM_001382
• Insert Size: 496 bp
• Sequence Data:
  Insert Sequence

  >SC206778 3'UTR clone of NM_001382
  The sequence shown below is from the reference sequence of NM_001382. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCAGCTCGTTCGACTCTTCTATGATGTCTGAGTCCCTTGATCATTGTCCTTTACCTCACAGTCTCTAGGA
  TTCCTGACTCAGGCTGACCTCTCTCTCTGGTCCCAGACTGCCTCCTTGCCCAGGCCTCTCTCACTCTTCA
  TACTCCTCCAGATTTTGTTCTCAGCATTTTCCTTTCTCTGTGATCATTGGCATCCTGGGCGTTTCTTGCC
  CTCTGCTGACTACTGATTGGATTTTACCTATGGCTTTCTGCAACTTGCTACTCTCTCCCTCTCCATCCCA
  TCTTTGCAGCCTCATAGGGTGGGATACAGCAGCTTTTTTTGCAGTTATCCACACTCACATTTCAGAGTCC
  TGACTCTCAAGGAACCACTGGTTTTTGGGATAGAACTTGGGCCAGGGCTAGGAACACAGGCTCCACGGTG
  ACATGTCATTTGATTGTAAATTAAGTGTTCTGATTAGTAAGAACTAAGCAGGGGGCCACATGCTCTCAAT
  GGAGAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001382.3
• Summary: 'The protein encoded by this gene is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic reticulum. The congenital disorder of glycosylation type Ij is caused by mutation in the gene encoding this enzyme. [provided by RefSeq, Jul 2008]'
• Locus ID: 1798