PRP18 homolog (PRPF18) (NM_003675) Human 3' UTR Clone

CAT#: SC207169

3`UTR clone of PRP18 pre-mRNA processing factor 18 homolog (S. cerevisiae) (PRPF18) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PRPF18
• Synonyms: hPrp18; PRP18
• ACCN: NM_003675
• Insert Size: 523
• Sequence Data:
  Insert Sequence

  >SC207169 3'UTR clone of NM_003675
  The sequence shown below is from the reference sequence of NM_003675. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTGTGGAGTACAATGCACTGTGAGATCTGTGTATGGTGTGTTAATAACAATAAGAAACTTAGGGAAGCAG
  GCTGTGGACTTCTGGAATTACCAACAGGAATGAGGAAAGAAGAAAACTGGAGTTTCCAGTCTCTGAGTTC
  TACCTGATGTAACTCTTGATTGGTTTTAAGAACTTTGTTGGCCTTCATTTCATATCTGACTGCAAGCTGA
  TTTTTCTTTCTTGCTTTCATTTTAATTAGTCCAAAATTAAGTTTTAAAGATTTTTCCTCACAATTTAAAT
  CCATAGACAACAGAAGGGGGTTTAAAATGACCTTTTTTTCAGTTGACCCGAAAGTTGTGGTTAGATGATT
  AAAAAGAAACATTTGAGGATGGACTTAATTATTTCAGTAAAATATTATAATTTGCCTATTGTCTTTTATG
  TAATATTTATATAAAATATTTTTATATATTTCAAAAACATGGGACCATTGAATGAAACTTTATAGTCCTT
  AAATGTTGCTGAACTTTTGCTGTCTTGCAATAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_003675.3
• Summary: Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. [provided by RefSeq, Jul 2008]
• Locus ID: 8559