NPL (NM_030769) Human 3' UTR Clone

CAT#: SC207307

3`UTR clone of N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NPL
• Synonyms: C1orf13; C112; NAL; NPL1
• ACCN: NM_030769
• Insert Size: 539
• Sequence Data:
  Insert Sequence

  >SC207307 3'UTR clone of NM_030769
  The sequence shown below is from the reference sequence of NM_030769. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGAAACTTGGAAGCTGGTAGCTAGTGCCTCTCTATCAAATCAGGGTTTGCACCTTGAGACATAATCTACC
  TTAAATAGTGCATTTTTTTCTCAGGGAATTTTAGATGAACTTGAATAAACTCTCCTAGCAAATGAAATCT
  CACAATAAGCATTGAGGTACCTTTTGTGAGCCTTAAAAAGTCTTATTTTGTGAAGGGGCAAAAACTCTAG
  GAGTCACAACTCTCAGTCATTCATTTCACAGATTTTTTTGTGGAGAAATTTCTGTTTATATGGATGAAAT
  GGAATCAAGAGGAAAATTGTAATTGATTAATTCCATCTGTCTTTAGGAGCTCTCATTATCTCGGTCTCTG
  GTTCCTAATCCTATTTTAAAGTTGTCTAATTTTAAACCACTATAATATGTCTTCATTTTAATAAATATTC
  ATTTGGAATCTAGGAAAACTCTGAGCTACTGCATTTAGGCAGGCACTTTAATACCAAACTGTAACATGTC
  TCAACTGTATACAACTCAAAATACACCAGCTCATTTGGCTGCTCAGTCT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_030769.1
• Summary: This gene encodes a member of the N-acetylneuraminate lyase sub-family of (beta/alpha)(8)-barrel enzymes. N-acetylneuraminate lyases regulate cellular concentrations of N-acetyl-neuraminic acid (sialic acid) by mediating the reversible conversion of sialic acid into N-acetylmannosamine and pyruvate. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
• Locus ID: 80896