BRMS1 (NM_015399) Human 3' UTR Clone

CAT#: SC207572

3`UTR clone of breast cancer metastasis suppressor 1 (BRMS1) transcript variant 1 for miRNA target validation


Reconstitution Protocol

USD 560.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol BRMS1
Synonyms DKFZp564A063
ACCN NM_015399
Insert Size 542
Sequence Data
>SC207572 3'UTR clone of NM_015399
The sequence shown below is from the reference sequence of NM_015399. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

AAATCGGATGGACCTTGACCCTGCTGTTCACAGCCAGGGGGACCCTCAGAGCAGCTGGCACTGCACCCAG
GATTCTCGTCTTCCTCCTGCAGACAGGCGGACCCACAGGCCCCTCAGGGTCTGCCCAGCCAGGCTCCTGT
GGTGCTGCTGGGCCCTCCCACTCCATCTGGCACTGGCCTGGACTCCTCCTCTGCCCTCCTCGAGGCCTGC
ACAGCTGTGGCCGTGGAGCTGACCTGACCAGGCAAGGCTGCTGTCTCCATCCCTGAGCCGCCTGCCACCT
CCCACTCCTGAAGATCCATCTCTTGGGGCTCCCCTGACAGAGAAGACAGCCGAAGTCAAAGCCACATCCT
CTTGCTGATGTTGGATGCAGGCTGTCCGGCCTCAGGGCCAGGGAGCCAGTTTCCACTGTGCGGGAACTCT
GAGTCAGACGTGATTATCTGGGGGTCTGTCCACCCTGGCTGGATCTGGAGGCAAGATGCCAGGCCCCCCA
GGTGTTCTCAGGGCAGTTCTTGGTGTCTGCTTCTCAGATTCCAAGGACTGGA

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq NM_015399.3
Summary This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Locus ID 25855

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