FUCA2 (NM_032020) Human 3' UTR Clone

CAT#: SC207967

3`UTR clone of fucosidase alpha-L- 2 plasma (FUCA2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: FUCA2
• Synonyms: dJ20N2.5
• ACCN: NM_032020
• Insert Size: 612 bp
• Sequence Data:
  Insert Sequence

  >SC207967 3'UTR clone of NM_032020
  The sequence shown below is from the reference sequence of NM_032020. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTCTAGCCCTGACTAATGTGATCTAAAGTGCAGCAGAGTGGCTGATGCTGCAAGTTATGTCTAAGGCTAG
  GAACTATCAGGTGTCTATAATTGTAGCACATGGAGAAAGCAAATGTAAAACTGGATAAGAAAATTATTTT
  GGCAGTTCAGCCCTTTCCCTTTTTCCCACTAAATTTTTTCTTAAATTACCCATGTAACCATTTTAACTCT
  CCAGTGCACTTTGCCATTAAAGTCTCTTCACATTGATTTGTTTCCATGTGTGACTCAGAGGTGAGAATTT
  TTTCACATTATAGTAGCAAGGAATTGGTGGTATTATGGACCGAACTGAAAATTTTATGTTGAAGCCATAT
  CCCCCATGATTATATAGTTATGCATCACTTAATATGGGGATATTTTCTGGGAAATGCATTGCTAGTCAAT
  TTTTTTTTGTGCCAACATCATAGAGTGTATTTACAAAATCCTAGATGGCATAGCCTACTACACACCTAAT
  GTGTATGGTATAGACTGTTGCTCCTAGGCTACAGACATATACAGCATGTTACTGAATACTGTAGGCAATA
  GTAACAGTGGTATTTGTATATCGAAACATATGGAAACATAGAGAAGGTACAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_032020.3
• Summary: 'This gene encodes a plasma alpha-L-fucosidase, which represents 10-20% of the total cellular fucosidase activity. The protein is a member of the glycosyl hydrolase 29 family, and catalyzes the hydrolysis of the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. This enzyme is essential for Helicobacter pylori adhesion to human gastric cancer cells. [provided by RefSeq, Aug 2010]'
• Locus ID: 2519