NAGPA (NM_016256) Human 3' UTR Clone

CAT#: SC208489

3`UTR clone of N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NAGPA
• Synonyms: APAA; UCE
• ACCN: NM_016256
• Insert Size: 667
• Sequence Data:
  Insert Sequence

  >SC208489 3'UTR clone of NM_016256
  The sequence shown below is from the reference sequence of NM_016256. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAGAGAAGGAGCAGCCAGGGGGCGCCCACAACCCCTTCAAGGACTGAAGCCTCAAGCTGCCCGGGGTGGC
  ACGTCGCGAAAGCTTGTTTCCCCACGGTCTGGCTTCTGCAGGGGAAATTTCAAGGCCACTGGCGTGGACC
  ATCTGGGTGTCCTCAGCCCCTGTGGGGCAGCCAAGTTCCTGATAGCACTTGTGCCTCAGCCCCTCACCTG
  GCCACCTGCCAGGGCACCTGCAACCCTAGCAATACCATGCTCGCTGGAGAGGCTCAGCTGCCTGCTTCTG
  GCCTGCCTGTGTCTGCTGCCGAGAAGCCCGTGCCCCCGGGAGGGCTGCCGCACTGCCAAAGAGTCTCCCT
  CCTCCTGGGGAAGGGGCTGCCAACGAACCAGACTCAGTGACCACGTCATGACAGAACAGCACATCCTGGC
  CAGCACCCCTGGCTGGAGTGGGTTAAAGGGACGAGTCTGCCTTCCTGGCTGTGACACGGGACCCCTTTTC
  TACAGACCTCATCACTGGATTTGCCAACTAGAATTCGATTTCCTGTCATAGGAAGCTCCTTGGAAGAAGG
  GATGGGGGGATGAGATCATGTTTACAGACCTGTTTTGTCATCCTGCTGCCAAGAAGTTTTTTAATCACTT
  GAATAAATTGATATAATAAAAGGAGCCACCAGGTGGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_016256.2
• Summary: Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. The encoded preproprotein is proteolytically processed by furin to generate the mature enzyme, a homotetramer of two disulfide-linked homodimers. Mutations in this gene are associated with developmental stuttering in human patients. [provided by RefSeq, Oct 2015]
• Locus ID: 51172