PCK1 (NM_002591) Human 3' UTR Clone

CAT#: SC208602

3`UTR clone of phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PCK1
• Synonyms: PCKDC; PEPCK-C; PEPCK1; PEPCKC
• ACCN: NM_002591
• Insert Size: 663 bp
• Sequence Data:
  Insert Sequence

  >SC208602 3'UTR clone of NM_002591
  The sequence shown below is from the reference sequence of NM_002591. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAGATCCTTGCCTTGAAGCAAAGAATAAGCCAGATGTAATCAGGGCCTGAGTGCTTTACCTTTAAAATCA
  TTCCCTTTCCCATCCATAAGGTGCAGTAGGAGCAAGAGAGGGCAAGTGTTCCCAAATTGACGCCACCATA
  ATAATCATCACCACACCGTGAGCAGATCTGAAAGGCACACTTTGATTTTTTTAAGGATAAGAACCACAGA
  ACACTGGGTAGTAGCTAATGAAATTGAGAAGGGAAATCTTAGCATGCCTCCAAAAATTCACATCCAATGC
  ATAGTTTGTTCAAATTTAAGGTTACTCAGGCATTGATCTTTTCAGTGTTTTTTCACTTTAGCTATGTGGA
  TTAGCTAGAATGCACACCAAAAAAATACTTGAGCTGTATATATATATGTGTGTGTGTGTGTGTGTGTGTG
  TGTGTGTGTGTGCATGTATGTGCACATGTGTCTGTGTGGTATATTTGTGTATGTGTATTTGTATGTACTG
  TTATTGAAAATATATTTAATACCTTTGGAAAAATCTTGGGCAAGATGACCTACTAGTTTTCCTTGAAAAA
  AAGTTGCTTTGTTATTAATATTGTGCTTAAATTATTTTTATACACCATTGTTCCTTACCTTTACATAATT
  GCAATATTTCCCCCTTACTACTTCTTGGAAAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002591.3
• Summary: 'This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008]'
• Locus ID: 5105