Folylpolyglutamate synthase (FPGS) (NM_004957) Human 3' UTR Clone

CAT#: SC208753

3`UTR clone of folylpolyglutamate synthase (FPGS) nuclear gene encoding mitochondrial protein transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: FPGS
• Synonyms: folylpoly-gamma-glutamate synthetase; folylpolyglutamate synthase; folylpolyglutamate synthetase; OTTHUMP00000022201; OTTHUMP00000022202; tetrahydrofolylpolyglutamate synthase
• ACCN: NM_004957
• Insert Size: 712 bp
• Sequence Data:
  Insert Sequence

  >SC208753 3'UTR clone of NM_004957
  The sequence shown below is from the reference sequence of NM_004957. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGGTGTCCTGAAGCTGCTGGAGCCCGCACTGTCCCAGTAGCCAAGGCCCGGGGTTGGAGGTGGGAGCTTC
  CCACACCTGCCTGCGTTCTCCCCATGAACTTACATACTAGGTGCCTTTTGTTTTTGGCTTTCCTGGTTCT
  GTCTAGACTGGCCTAGGGGCCAGGGCTTTGGGATGGGAGGCCGGGAGAGGATGTCTTTTTTAAGGCTCTG
  TGCCTTGGTCTCTCCTTCCTCTTGGCTGAGATAGCAGAGGGGCTCCCCGGGTCTCTCACTGTTGCAGTGG
  CCTGGCCGTTCAGCCTGTCTCCCCCAACACCCCGCCTGCCTCCTGGCTCAGGCCCAGCTTATTGTGTGCG
  CTGCCTGGCCAGGCCCTGGGTCTTGCCATGTGCTGGGTGGTAGATTTCCTCCTCCCAGTGCCTTCTGGGA
  AGGGAGAGGGCCTCTGCCTGGGACACTGCGGGACAGAGGGTGGCTGGAGTGAATTAAAGCCTTTGTTTTT
  TAAAGAAATGGCAAAGCCTTCGACTGACCCTTGACCCCCTGCTCCCTCAGCAGAGACGGAGGGAGGGGCT
  GCTGGTGGTCAGGGACCTGCACTGTGTAGAGGGAGCCTGGCTGTGTGGCCTGGAACAAGTCCCTCCCTCC
  CTGTGCGCCTCAGGTGGCCTGTCTGTGAGATGAGAAGAAGACCAGACTGAAGCCTGTTCACCATATGCCA
  GGCAGTGCTTTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004957.4
• Summary: 'This gene encodes the folylpolyglutamate synthetase enzyme. This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. This enzyme catalyzes the ATP-dependent addition of glutamate moieties to folate and folate derivatives. Alternative splicing results in transcript variants encoding different isoforms. [provided by RefSeq, Jan 2014]'
• Locus ID: 2356