Aspartate Aminotransferase (GOT1) (NM_002079) Human 3' UTR Clone

CAT#: SC208953

3`UTR clone of glutamic-oxaloacetic transaminase 1 soluble (aspartate aminotransferase 1) (GOT1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GOT1
• Synonyms: AST1; ASTQTL1; cAspAT; cCAT; GIG18
• ACCN: NM_002079
• Insert Size: 680 bp
• Sequence Data:
  Insert Sequence

  >SC208953 3'UTR clone of NM_002079
  The sequence shown below is from the reference sequence of NM_002079. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCATCCATGAAGCAGTCACCAAAATCCAGTGAAGAAACACCACCCGTCCAGTACCACCAAAGTAGTTCTC
  TGTCATGTGTGTTCCCTGCCTGCACAAACCTACATGTACATACCATGGATTAGAGACACTTGCAGGACTG
  AAAGGCTGCTCTGGTGAGGCAGCCTCTGTTTAAACCGGCCCCACATGAAGAGAACATCCCTTGAGACGAA
  TTTGGAGACTGGGATTAGAGCCTTTGGAGGTCAAAGCAAATTAAGATTTTTATTTAAGAATAAAAGAGTA
  CTTTGATCATGAGACATAGGTATCTTGTCCCTCTCACTAAAAAGGAGTGTTGTGTGTGGCGGCCACGTGC
  TTCTATGTGGTGTTTGACTCTGTACAAATTCTAGTCCCAAAGATCAAGTTGTCTGAAGGAGCCAAAGTGT
  GAATGTGGGTGTCGGCTGCGGCATTAAATTCATCATCTCAACCCAGAGTGTCTGGTCTCCCTGCTCTTTC
  TGCATGGTTGTGTCCCTAGTCCTAAGCTTTGGTTCTTTAGGGTGACTGTGGTAAGAAGGATATTTAATCA
  TGACATGCACGGACACGTACATATTTAACTGAAACAAGTTTTACCAAACAGTATTTACTCGTGATGTGCG
  TAGTGCATTCTGATATTTTTGAGCCATTCTATTGTGTTCTACTTCACCTA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002079.2
• Summary: 'Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]'
• Locus ID: 2805