CBS (NM_000071) Human 3' UTR Clone

CAT#: SC209154

3`UTR clone of cystathionine-beta-synthase (CBS) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CBS
• Synonyms: CBSL; HIP4
• ACCN: NM_000071
• Insert Size: 709 bp
• Sequence Data:
  Insert Sequence

  >SC209154 3'UTR clone of NM_000071
  The sequence shown below is from the reference sequence of NM_000071. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GACTTGCTGAACTTCGTGGCCGCCCAGGAGCGGGACCAGAAGTGAAGTCCGGAGCGCTGGGCGGTGCGGA
  GCGGGCCCGCCACCCTTGCCCACTTCTCCTTCGCTTTCCTGAGCCCTAAACACACGCGTGATTGGTAACT
  GCCTGGCCTGGCACCGTTATCCCTGCACACGGCACAGAGCATCCGTCTCCCCTCGTTAACACATGGCTTC
  CTAAATGGCCCTGTTTACGGCCTATGAGATGAAATATGTGATTTTCTCTAATGTAACTTCCTCTTAGGAT
  GTTTCACCAAGGAAATATTGAGAGAGAAGTCGGCCAGGTAGGATGAACACAGGCAATGACTGCGCAGAGT
  GGATTAAAGGCAAAAGAGAGAAGAGTCCAGGAAGGGGCGGGGAGAAGCCTGGGTGGCTCAGCATCCTCCA
  CGGGCTGCGCCGTCTGCTCGGGGCTGAGCTGGCGGGAGCAGTTTGCGTGTTTGGGTTTTTTAATTGAGAT
  GAAATTCAAATAACCTAAAAATCAATCACTTGAAAGTGAACAATCAGCGGCATTTAGTACATCCAGAAAG
  TTGTGTAGGCACCACCTCTGTCACGTTCTGGAACATTCTGTCATCACCCCGTGAAGCAATCATTTCCCCT
  CCCGTCTTCCTCCTCCCCTGGCAACTGCTGATCGACTTTGTGTCTCTGTTGTCTAAAATAGGTTTTCCCT
  GTTCTGGAC
  
  CGGACCGTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000071.2
• Summary: 'The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. This gene is a major contributor to cellular hydrogen sulfide production. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2016]'
• Locus ID: 875