FDFT1 (NM_004462) Human 3' UTR Clone

CAT#: SC209159

3`UTR clone of farnesyl-diphosphate farnesyltransferase 1 (FDFT1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: FDFT1
• Synonyms: DGPT; ERG9; SQS; SQSD; SS
• ACCN: NM_004462
• Insert Size: 742 bp
• Sequence Data:
  Insert Sequence

  >SC209159 3'UTR clone of NM_004462
  The sequence shown below is from the reference sequence of NM_004462. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTCCCAGGTAACAGAAGACTATGTTCAGACTGGAGAACACTGATCCCAAATTTGTCCATAGCTGAAGTCC
  ACCATAAAGTGGATTTACTTTTTTTCTTTAAGGATGGATGTTGTGTTCTCTTTATTTTTTTCCTACTACT
  TTAATCCCTAAAAGAACGCTGTGTGGCTGGGACCTTTAGGAAAGTGAAATGCAGGTGAGAAGAACCTAAA
  CATGAAAGGAAAGGGTGCCTCATCCCAGCAACCTGTCCTTGTGGGTGATGATCACTGTGCTGCTTGTGGC
  TCATGGCAGAGCATTCAGTGCCACGGTTTAGGTGAAGTCGCTGCATATGTGACTGTCATGAGATCCTACT
  TAGTATGATCCTGGCTAGAATGATAATTAAAAGTATTTAATTTGAAGCACCATTTGAATGTTCGTAATAG
  TAGAAAATGATGTGAATTTTCTTTCTGTTCGGCTCCTATTTTTCTCATCATTTTGTTTTCTTTAATTGGG
  TTGAATGGAGTAGATAGAAATATTTATGGTTTAGGTAACAGTTAGATGTTTCCTAAGAATGCAAACTGCC
  TTTTCCACACAAAGGCTGGGAATAAAATTCTGGGTATTCTCGTATTCTCATTTAAAGGAGTTTAGCTTTC
  AGAGAGAAACAGCAGGATTGCTTTTGACCTTTTAGAAGATTGGTCTCCAGTAAAGGTGGACATTTTTGAG
  ATTTTTATAATAAAGAATTTAATTGCTCTGCATTTGTCAAGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004462.3
• Summary: 'This gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. [provided by RefSeq, Jul 2008]'
• Locus ID: 2222