Perforin (PRF1) (NM_001083116) Human 3' UTR Clone
CAT#: SC209308
3`UTR clone of perforin 1 (pore forming protein) (PRF1) transcript variant 2 for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | PRF1 |
Synonyms | HPLH2; P1; PFP |
ACCN | NM_001083116 |
Insert Size | 719 bp |
Sequence Data |
>SC209308 3'UTR clone of NM_001083116
The sequence shown below is from the reference sequence of NM_001083116. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon CAATTGGCAGAGCTCAGAATTCAAGCGATCGC GGAGCCTCCAGGAAACCGGAGTGGGGCCGTGTGGTGAGAACAGTGAGCTTGGAAAGGACCAGTATGCTTG GACTGAAGGGGTTCTCACAGTGGGAGCCAGGGCTGTCTTCGTATTCCCATTAGACCAAGCTTGTCCAACC CGAGGCCCGCATGCGGCCCAGGATGGCTTTGAATGCGGCCCAACGCAAATTCGCAAACTTTCTTAAAACA TTATGAGTTTCTTTTTGCTATTTTTTTTTTTTTTTTAGCTCATCGGCTATCGTTAGTGCTAGTGGATTTT ACATGTGGCCCAACACAATTCTTCTTCCAACGTGGCCCAGAGAAGCCAAAAGATTGGATACGCATCAGAC AGATGGAAAAGGGAGATTCAGACTGTTTTTCAGGGAGGTGGCTGGGTTTACACGCTAATCCCGATTCACC CTGTCCAAACTGCCTAAGCCCTCCGCCATTCTCAAGCCCTGCAGTCACAGCTACACAGATCACAGCTTCA GCCAGGAGCTGGGCAGAAGGCCAAGAGGCTGTTCCCACCAGGCTGCTCAGGGCTGGTCTTTTAGGACCCT TCCCTTGAGCCCTCTATGGTGTGGCAAAGCCTTCATTGCCTTAACTGGAGCCCCATCAGCTCCAGCTGCT CTGTCTTCTTTGCCCACAATGCTTTGCCCCTGAGACAAATGGAGGCCTGTCCTGACCTGTCTCACCATGT ACATAGCTTGATAAAGGGC ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Reference Data | |
RefSeq | NM_001083116.1 |
Summary | 'This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017]' |
Locus ID | 5551 |
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