Selenophosphate synthetase 2 (SEPHS2) (NM_012248) Human 3' UTR Clone

CAT#: SC209703

3`UTR clone of selenophosphate synthetase 2 (SEPHS2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SEPHS2
• Synonyms: SPS2; SPS2b
• ACCN: NM_012248
• Insert Size: 788
• Sequence Data:
  Insert Sequence

  >SC209703 3'UTR clone of NM_012248
  The sequence shown below is from the reference sequence of NM_012248. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCTGACAGTTCAAATGCCTCCTCTGAGCCTAGCTCGTGAGATGAAAGAACAGAAGTTGTTTGGACCTTAG
  AGCCATTGTCCACAATCACGGATGGTTCTCAAGAGTTGATTGTAAGAAATTTCCAAAGAAGGCTGCCTGC
  ATAGTGGTTCCGGCTGCCCTTTCTAGGTGATTGGAATCAGCCCATCTAAAGCAGTCTTTATATGCATTCC
  GAGGCCAGAGTAACATTTTGAACTTTGGGGGGATATTTGTTCATCACTTGGGTAGAAGAGGAGCAAAAAT
  ACCTCTGTTTTCTCTTGCCAAAGTAAGATGAAGCTATTCCAGGTTGAGGGATTTTTCTTTGCACGGGGTT
  GATTAATTTCTGCACAGGGAGTGAGATTATTAAAGTAACACACACACAAAGTAAATTGCAAAATGAAAAA
  AATTAGAAGCAAATGAGTTTTGGACCAATATTGTTGATAAATCTAAATTGTTAAGAGAGATCTTATAATG
  CAACATCAAATTCTTTATTCAATTTTACTGAAGTACTGGCTCTTTCCTGCTCTGGACAAGAATTGAGCAA
  CTTGTCTGATGACTGGGAAAGGAGGACCTGCAACCATCTGACTTGGTCTCTGTTAATGACGTCTCTCCCT
  CTAAACCCCATTAAGGACTGGGAGAGGCAGAGCAAGCCTCAGAGCCCAGGCCTCAGTGGTCATTAAGATG
  TTAAGTCTTTTGCGGCAGATTCCTGGTGATTTGATCAATAAAGAGTAATTTCTTGCTAAATAAATAAAAG
  AAACCTTGTTGAAAAACT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_012248.2
• Summary: This gene encodes an enzyme that catalyzes the production of monoselenophosphate (MSP) from selenide and ATP. MSP is the selenium donor required for synthesis of selenocysteine (Sec), which is co-translationally incorporated into selenoproteins at in-frame UGA codons that normally signal translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is itself a selenoprotein containing a Sec residue at its active site, suggesting the existence of an autoregulatory mechanism. It is preferentially expressed in tissues implicated in the synthesis of selenoproteins and in sites of blood cell development. A pseudogene for this locus has been identified on chromosome 5. [provided by RefSeq, May 2017]
• Locus ID: 22928