HHIP (NM_022475) Human 3' UTR Clone

CAT#: SC209741

3`UTR clone of hedgehog interacting protein (HHIP) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HHIP
• Synonyms: HIP
• ACCN: NM_022475
• Insert Size: 812
• Sequence Data:
  Insert Sequence

  >SC209741 3'UTR clone of NM_022475
  The sequence shown below is from the reference sequence of NM_022475. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GACATCTTACTTGCTGGATCTAACAAGTTACATTGTATAGTTTCTGGGACTGTTTGAATATTCTATTCCA
  ATGGGCATTTATTTTTTATCCTGTCATTAAAAAAAAAAGACTGTTATCCTGCTACACACTCCTGTGATTT
  CATTCTCTTTTATTAATTTAAAAATAATTTCCAGAAATGTGCAGATCCTCTGTGTGTATGTCAGCATGTT
  TGTTCACATATGCACATACACATACTCATAACCCCTATATGCGTTGTTGCATAACAGATGATTTTTTAAA
  ATATATACTTCCTTATGCAAAGTAATTTACACAGAAATTCCATTGTAAATTGATAATGGATTTTTTATGT
  TACTAGAAGAGATTATTTGACTTCCCAGGAATTTTCTGTCTGTAATCACTAAAGTCAACTTTAATAGAGT
  TTTGAAACAGTACTGTGCAATCCGATGGATCTAATTAAAAAAAAGGCAATATTTTTATATTAAAGTACTA
  TACTAGGAGAGAATGTTTCAGAACTCCCTGATGAATTTCTAAGTGAGCAACTTGATATAAAATTGTAATC
  TTCATTTTTGTCAGTGTATCCAGTTACAGAATGCTACACACTTACCTTTTTATTGGCTGAGAAATCTGGT
  TATTTCATCTTAATCTCAAGATTGTTTTCAAGTGTTTTATAATTAAATCATAATAGCATATTTTAAAATC
  AATCTTCCTAAAAGGTCTGCTTTTATTGTATATTTTATTTAACAATAGGCACTGGGTTTGTGTTACATAT
  TTATATATTTTATTTTATTTTTATAATATAGACATCACCTAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_022475.1
• Summary: This gene encodes a member of the hedgehog-interacting protein (HHIP) family. The hedgehog (HH) proteins are evolutionarily conserved protein, which are important morphogens for a wide range of developmental processes, including anteroposterior patterns of limbs and regulation of left-right asymmetry in embryonic development. Multiple cell-surface receptors are responsible for transducing and/or regulating HH signals. The HHIP encoded by this gene is a highly conserved, vertebrate-specific inhibitor of HH signaling. It interacts with all three HH family members, SHH, IHH and DHH. Two single nucleotide polymorphisms (SNPs) near this gene are significantly associated with risk of chronic obstructive pulmonary disease (COPD). A single nucleotide polymorphism in this gene is also strongly associated with human height. [provided by RefSeq, Feb 2011]
• Locus ID: 64399