NFYC (NM_001142587) Human 3' UTR Clone

CAT#: SC209914

3`UTR clone of nuclear transcription factor Y gamma (NFYC) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NFYC
• Synonyms: CBF-C; CBFC; H1TF2A; HAP5; HSM; NF-YC
• ACCN: NM_001142587
• Insert Size: 763 bp
• Sequence Data:
  Insert Sequence

  >SC209914 3'UTR clone of NM_001142587
  The sequence shown below is from the reference sequence of NM_001142587. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGTGACCGGCGACTGAGGGCCTGAGCTGGCAAGGCCAAGGACACCCAACACAATTTTTGCCATACAGCC
  CCAGGCAATGGGCACAGCCTTCCTCCCCAGAGGACCCGGCCGACCTCAGCGCCTCCTGCAGGCTAGGACA
  CTGGTGCACTACACCCCATGCCTGGGGGCCGAGATTCTCCAGCAGAAAGATGCAATATTTTTTGTTTCCT
  TTTTTTCCATTTTTTTCTCTAAGGAATCAATATTTCAATATGTTGAGTGTGTGTCCAATGCTATGAAATT
  AAAATATTAAATAACATATTTATGGCATTTTCTTGAAGAGTGTGGTTGAAGAAATATTTCTCCTTTTGTT
  TTTCTTTTTTTTTTGTTTGTTACTGCCACTTCTTTTTAGGAGCAAATCTCCCCAGGGGTGTACGGTATTT
  CTTGACTCTGGGAACAGCTGCTACCCCCAAGACTTGCCACGTTGTTCTGCCCTCAGATGGAATTAGGTGA
  ATGTGTGTAGCTGCTTTTTCACTCGTGGTCCTCTCCCCATCCCTTGCTCTGACCCCAGAGCTCTGTGTAT
  TTGCATCCAGAGGCCATGGAAACATTCTTTGCATTTAAGAGACAGATTTATTCCCTGTGGAGAGTGGGTG
  GATTCATTGCCACACTCTTTTCTCCCAGGGACCCAGGAAACTAGGACTTTGTGTGTTTGCTGCCCACCTC
  CCTTTTATTTTTTAAATGCATTAAAAACTGTGCTAGTCTCCTTTGCATGGACTTCAAGCTGCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001142587.1
• Summary: 'This gene encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]'
• Locus ID: 4802