RASSF1 (NM_007182) Human 3' UTR Clone

CAT#: SC210114

3`UTR clone of Ras association (RalGDS/AF-6) domain family member 1 (RASSF1) transcript variant A for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RASSF1
• Synonyms: 123F2; NORE2A; RASSF1A; RDA32; REH3P21
• ACCN: NM_007182
• Insert Size: 819
• Sequence Data:
  Insert Sequence

  >SC210114 3'UTR clone of NM_007182
  The sequence shown below is from the reference sequence of NM_007182. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CGCCAGAAGATCCAAGAGGCCCTGCACGCCTGCCCCCTTGGGTGACCTCTTGTACCCCCAGGTGGAAGGC
  AGACAGCAGGCAGCGCCAAGTGCGTGCCGTGTGAGTGTGACAGGGCCAGTGGGGCCTGTGGAATGAGTGT
  GCATGGAGGCCCTCCTGTGCTGGGGGAATGAGCCCAGAGAACAGCGAAGTAGCTTGCTCCCTGTGTCCAC
  CTGTGGGTGTAGCCAGGTATGGCTCTGCACCCCTCTGCCCTCATTACTGGGCCTTAGTGGGCCAGGGCTG
  CCCTGAGAAGCTGCTCCAGGCCTGCAGCAGGAGTGGTGCAGACAGAAGTCTCCTCAATTTTTGTCTCAGA
  AGTGAAAATCTTGGAGACCCTGCAAACAGAACAGGGTCATGTTTGCAGGGGTGACGGCCCTCATCTATGA
  GGAAAGGTTTTGGATCTTGAATGTGGTCTCAGGATATCCTTATCAGAGCTAAGGGTGGGTGCTCAGAATA
  AGGCAGGCATTGAGGAAGAGTCTTGGTTTCTCTCTACAGTGCCAACTCCTCACACACCCTGAGGTCAGGG
  AGTGCTGGCTCACAGTACAGCATGTGCCTTAATGCTTCATATGAGGAGGATGTCCCTGGGCCAGGGTCTG
  TGTGAATGTGGGCACTGGCCCAGGTTCATACCTTATTTGCTAATCAAAGCCAGGGTCTCTCCCTCAGGTG
  TTTTTTATGAAGTGCGTGAATGTATGTAATGTGTGGTGGCCTCAGCTGAATGCCTCCTGTGGGGAAAGGG
  GTTGGGGTGACAGTCATCATCAGGGCCTGGGGCCTGAGAGAATTGGCTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_007182.4
• Summary: This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, May 2011]
• Locus ID: 11186