USP1 (NM_003368) Human 3' UTR Clone

CAT#: SC210290

3`UTR clone of ubiquitin specific peptidase 1 (USP1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: USP1
• Synonyms: UBP
• ACCN: NM_003368
• Insert Size: 860 bp
• Sequence Data:
  Insert Sequence

  >SC210290 3'UTR clone of NM_003368
  The sequence shown below is from the reference sequence of NM_003368. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACTTCTACTCCTTACTTGCTATTTTATAAGAAATTATAGAGTGAGTGTATTTTCCTTGTGTATATATTAA
  ACACACCCATACAAACATTGGTAAAGTTGATTACATCAAAGAATCTTTAGCTTATCTTTTGAAGCTACTG
  GATATTATTGGTCTCTCTAGGTTTTTATATAAATAGTGAAATTTGAATTACTGAAAACCATGTTAATTTT
  TAGAACTCATTTTCCTCAGTAGAGACTAGTGATGCATTAGCTTCTGGGAACAAACTTGTATCGGTTCTTA
  ATTAAATTATCCAAAACGGAGGCATTTAAACACTTGGATTTACACCAGTCTTTTGTGTTTGCTTTTTAAA
  ATAAAGTGCTCGTATTTGTATTCTCCATATTTTGGAGTAATTATCTACATGATGTTTATAGTTCCTGTGG
  TTTTTCACCCAAGAAGCAGAATCTCATTCAGTACATTTAGTTTTATAAGAGTCATGAAGCTAAATCCTTG
  GGCTATGTCAGAGGCACAAAGTCTAGAATGTGTGTATTCACAATGGTGTATGTACATTTTGTGCCTTGAT
  TCACTTAGAAGTGTCTCAGAAAACCTGGACAGTTCGCTTCTACACAAGAATTTTATATGTATTTATGAAG
  ATGATTCTGTACCCTAGTATATCTTTTTGGGCATGGACTAATTTGTATCTGTTTAACTCATATTCTGCAC
  GATCTGTATATAGTACATCAAACTTAGAGGTGTGACCTTAAATTTAACTTTTTTTAAAAACTGGGAGGTC
  AATAAAATTTAAACTGCTTAACTATGTATATGAATATTTGAATTTTTTACTTGTATATTTTTATAAATAC
  AGCTGAGTTTTCTTAAAGCG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003368.4
• Summary: 'This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. The protein specifically deubiquitinates a protein in the Fanconi anemia (FA) DNA repair pathway. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]'
• Locus ID: 7398