HMGCS2 (NM_005518) Human 3' UTR Clone

CAT#: SC210562

3`UTR clone of 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) (HMGCS2) nuclear gene encoding mitochondrial protein transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HMGCS2
• Synonyms: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial); hydroxymethylglutaryl-CoA synthase 2; OTTHUMP00000013928
• ACCN: NM_005518
• Insert Size: 859 bp
• Sequence Data:
  Insert Sequence

  >SC210562 3'UTR clone of NM_005518
  The sequence shown below is from the reference sequence of NM_005518. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CATCGCCGAAAGTATGCCCGGCGTCCCGTCTAAAGGTGTTCTGCAGATCCATGGAAAGCTTCCTGGGAAA
  CGTATGCTAGCAGAGCTTCTCCCCGTGAATCATATTTTTAAGATCCCACTCTTAGCTGGTAAATGAATTT
  GAATCGACATAGTAGCCCCATAAGCATCAGCCCTGTAGAGTGAGGAGCCATCTCTAGCGGGCCCTTCATT
  CCTCTCCATGCTGCAATCACTGTCCTGGGCTTATGGTGCTATGGACTAGGGGTCCTTTGTGAAAGAGCAA
  GATGGAGCAATGGAGAGAAGACCTCTTCCTGAATCACTGGACTCCAGAAATGTGCATGCAGATCAGCTGT
  TGCCTTCAAGATCCAGATAAACTTTCCTGTCATGTGTTAGAACTTTATTATTATTAATATTGTTAAACTT
  CTGTGCTGTTCCTGTGAATCTCCAAATTTTGTACCTTGTTCTAAGCTAATATATAGCAATTAAAAAGAGA
  GAAAGAGGAAATGATTCCTGCGTTTCTTGGAACCCAGAATACAAACCCAGCCTAACATGCAGCAAGCCTG
  CTAGACCTTGTGGGTCAGAGGGCTGGGTCCTTGCCTCACAGGCTGCCTCTGTCCCCTTGCAATTCCATTC
  TATTTCTGCCACATGCCAAGTGCTATGACAGGTACAAGGCAAATAAGAACGGTAGAACACAGCTTCCCCC
  AGCCCACTTCCCTGTTCTAAAGACACCACATAGACAGAGAGCAGCAGACAGGGGCCAGCAGGAGCTGTAG
  TTCAGATCTTCTTGGTCATTCCTTGCCGCTGTTATTTGAACAAATAAACACAGCGCAAAGGTTAACAAGT
  TTTTGCCTTCTATAGCCAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005518.3
• Summary: 'The protein encoded by this gene belongs to the HMG-CoA synthase family. It is a mitochondrial enzyme that catalyzes the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for various organs during times of carbohydrate deprivation, such as fasting. Mutations in this gene are associated with HMG-CoA synthase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]'
• Locus ID: 3158