GGA1 (NM_001001560) Human 3' UTR Clone

CAT#: SC210726

3`UTR clone of golgi associated gamma adaptin ear containing ARF binding protein 1 (GGA1) transcript variant 2 for miRNA target validation

Reconstitution Protocol

USD 560.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol GGA1
ACCN NM_001001560
Insert Size 869 bp
Sequence Data
>SC210726 3'UTR clone of NM_001001560
The sequence shown below is from the reference sequence of NM_001001560. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

GGGATGTGGACCAGTTCCCCCCACCTGAAACCTGGGGTAGCCTCTAGAACAGAGGGGCTGGGGAGAGGAA
GGGGCAGAGGGACCGGTCACTGTCCAGCCTGGAGGGAGGCATTGGTGGCCAAGGACACCCTTTGTTGCCC
ATGGCCATTCACCCCCAGGCCTGGTGCTTCTCCCCACACCCCTGTAGGCCTCAAGTGACTCTTCCCCCTC
CTGCTCCGGCCCCGCCCCTGCTGAGCCAAACCCAGTAGGAGGCTGGGCCTGGGTTTGTGCCGCTGGGGTC
TCCATCACCGGGACCTGGAGAGGGAGGGGCTGTGTAGCCTTGGAAGAACTTGGGTCATGGGGAGGAAGCA
CAGCTGTTGGGGAAGGGCCAGGACCTCAGGCCCAGCCCCAACCCCAGCTGGGGTGGGGTCTTCCCCACCT
GTCTCTTATGCCTTATGGGAAGGCCCAGCCATAACTCGGGGGCCATGCTGGAGCTGGGGACCAGCTTAGG
CCTCCTCCATAGGAACCCAGTGACTGGGGGGTGACGCCTACACCCCCAGCTATTTGCACTCTGGTGTGTG
GTTTGACTCTGCTTTTCTTCCGGATTGGCCCTGTGGTCACAGCCTCAGGGGGCCAGGCTGGGGGAACCTC
ACCTGGCCCGTACTCCTGGGGGTTTCCCTTTGCCATTGGGCCCCCTGAGGGACTGTGGGGGCTCAAGGGT
AATGCCAGAGGCCCATGGCCCCAGCGAGGGGCTGTGGGGCACCTAGAGTTCTCGGTGTGTCTCCTTCATT
CATTGGCCTCTGCTGGGGCCTCCTATGGGTGTCTTACGTCTGTCCATCCATCTGTCCGTGGTCAGAAGTG
GGGTCAGTGTGTGAGTGAGAGCAGGAGTA

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Reference Data
RefSeq NM_001001560.2
Summary This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Locus ID 26088

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.