MGAT2 (NM_002408) Human 3' UTR Clone

CAT#: SC210799

3`UTR clone of mannosyl (alpha-16-)-glycoprotein beta-12-N-acetylglucosaminyltransferase (MGAT2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MGAT2
• Synonyms: CDG2A; CDGS2; GLCNACTII; GNT-II; GNT2
• ACCN: NM_002408
• Insert Size: 908 bp
• Sequence Data:
  Insert Sequence

  >SC210799 3'UTR clone of NM_002408
  The sequence shown below is from the reference sequence of NM_002408. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGACCATGAACTCTGTAAAAGTTATAGAAGACTGCAGTGAAAATCACAGTTACAAAAGCGACAGTCTTCT
  ATTTTTGATATTTGTCCAAACAGGACATACAATTGAATAAAAGAGTTTAGGAACTGGTTTCTGCTTTAAT
  ACAAAAACAAAATCTTGTAAAAGGTGTCCAAATACATAGTAATCTTTTCCAGTTATGTCTGATTAAGATT
  TAAAACTGAAGGTTTCATTTTGGGAGTAGGGTTTTAAAGCTCAATCTGTTATCTGCTAAAATTGATTATT
  GTTGATATGAGAGAAGAGGGGAAATTTTATTTAAATTGCATTTATTAATCTTTTTATCTGAAACTTTGTA
  CACTTTTCCACTTTCAAAACCTATTTTAAGTACAGCAAAATTTATTTAAAACTGTCATAGCAGTAAAAAG
  TATTACGATGAAATTGTTAGGGTATTAATGGAACAAACCCAGTTTCACTCTCTTGACACACTTATTAGGA
  AGGGATTGCTTCACTGGTTTAATAATTTAAAAGTTATGTTTGTTAAACACCCTGTCAGAACAGTCATTTT
  CAGTATTAGATTCCTGTACTATTGTGTTTTGAGTGTGTTTTGGAACCTTCATAGAACACACTTTCTTTTG
  GAATGTATTTGATTGATAAGAAAGTTTAAACATTGTTTTCACCTCAATGTAGAAATACAGTGGTTTTGTT
  TTTTTTTTTCTTTTAGTGCTGACAAAATAAAATACTCATTTTTGCATAAAAAGGTTCCTAATCCTTTTGC
  AGAATAAGTTTTGTTTACTCTTTATACCAAAATTCAGTGAAGGCATTCTACAAGTTTTGAGTTAGCATTA
  CATTTTAATATTTACTATTGCTACATTGTATAATTGAGTTTGAAATAAAACCCAGCTTATGACAATGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002408.3
• Summary: 'The product of this gene is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. The coding region of this gene is intronless. Transcript variants with a spliced 5' UTR may exist, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]'
• Locus ID: 4247