SHMT1 (NM_148918) Human 3' UTR Clone

CAT#: SC210951

3`UTR clone of serine hydroxymethyltransferase 1 (soluble) (SHMT1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SHMT1
• Synonyms: CSHMT; SHMT
• ACCN: NM_148918
• Insert Size: 903 bp
• Sequence Data:
  Insert Sequence

  >SC210951 3'UTR clone of NM_148918
  The sequence shown below is from the reference sequence of NM_148918. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCTCTTCCCTCTGCCTGGCCTGCCTGACTTCTAAAGGAGCGGGCCCACTCTGGACCCACCTGGCGCCACA
  GAGGAAGCTGCCTGCCGGAGGACCCCCACCTGAGAGATGGATGAGCTGCTCCAAAGGGGAACTGTTGACA
  CTCGGGCCCTTTGAGGGGGTTTCTTTTGGACTTTTTTCATGTTTTCTTCACAAATCAAAATTTGTTTAAG
  TCTCATTGTTAGTAATTCTGGGACAGGTTATTAAAGGATTTAAATTTGAACCTGGCTTTCTCACAGCTGG
  ACATAATTCTAGGAAAATAAAATACTATGTCGCCACTTGGTCATAATCATTTAGATGGTGGTGTAGGGCA
  AAGCTGTTAGAAAGATTGTAGCGTTTTACTCTCCCTGGGCTTTCCTCCGCCTTGCTGCAACAGAGAGGAA
  ATGCCCATGTCCACAGCTTGTACACACTGCCCCCTCACTATCTTGTTATCCAGTGGCATGCCAAAGGAGA
  ACTGAATTAGCTTCTGAGGCTTCTGCTGTAAATCAGAAGTGTATGTTAGTCAAGAGTAAACAAGATGCAC
  CCAGTATGGTGGGAGGGTTTTGCTGTCAGTAGCTCAAAGTATGGTGTAGAAATGGCCTCCTCCCTCCATC
  CTGGGAAGTCCCAGTCCCATCCTGGTGTGAGAATCAACCAGGCTTTCCTGCTCCACCTGAGATAACCAAC
  TCCCTCCCGTAATCAGGAAGCCAAATGTCACCTTCCCAAAGAAATTTTATTTTCACGTAGCTGAAGTGCA
  AAACATAGATGACCATTTTTAATAAGCACAATCAAATTTTTAACCACAGAATGTCTACAAGAATTATAGC
  TTTAAAAAATACAACCAATTTTTATATTTCAAAAATATTTGAACTCAAATAAATTAATTTCTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_148918.1
• Summary: 'This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]'
• Locus ID: 6470