ACADM (NM_001127328) Human 3' UTR Clone

CAT#: SC211093

3`UTR clone of acyl-Coenzyme A dehydrogenase C-4 to C-12 straight chain (ACADM) nuclear gene encoding mitochondrial protein transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ACADM
• Synonyms: ACAD1; MCAD; MCADH
• ACCN: NM_001127328
• Insert Size: 919 bp
• Sequence Data:
  Insert Sequence

  >SC211093 3'UTR clone of NM_001127328
  The sequence shown below is from the reference sequence of NM_001127328. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGCCCGTGAACACATTGACAAGTACAAAAATTAAAAAAATTACTGTAGAAATATTGAATAACTAGAACAC
  AAGCCACTGTTTCAGCTCCAGAAAAAAGAAAGGGCTTTAACGTTTTTTCCAGTGAAAACAAATCCTCTTA
  TATTAAATCTAAGCAACTGCTTATTATAGTAGTTTATACTTTTGCTTAACTCTGTTATGTCTCTTAAGCA
  GGTTTGGTTTTTATTAAAATGATGTGTTTTCTTTAGTACCACTTTACTTGAATTACATTAACCTAGAAAA
  CTACATAGGTTATTTTGATCTCTTAAGATTAATGTAGCAGAAATTTCTTGGAATTTTATTTTTGTAATGA
  CAGAAAAGTGGGCTTAGAAAGTATTCAAGATGTTACAAAATTTACATTTAGAAAATATTGTAGTATTTGA
  ATACTGTCAACTTGACAGTAACTTTGTAGACTTAATGGTATTATTAAAGTTCTTTTTATTGCAGTTTGGA
  AAGCATTTGTGAAACTTTCTGTTTGGCACAGAAACAGTCAAAATTTTGACATTCATATTCTCCTATTTTA
  CAGCTACAAGAACTTTCTTGAAAATCTTATTTAATTCTGAGCCCATATTTCACTTACCTTATTTAAAATA
  AATCAATAAAGCTTGCCTTAAATTATTTTTATATGACTGTTGGTCTCTAGGTAGCCTTTGGTCTATTGTA
  CACAATCTCATTTCATATGTTTGCATTTTGGCAAAGAACTTAATAAAATTGTTCAGTGCTTATTATCATA
  TCTTTCTGTATTTTTTCCAGGAAATTTCATTACTTCGTGTAATAGTGTATATTTCTTGTATTTACTATGA
  TGAAAAAAGGTCGTTTTAATTTTGAATTGAATAAAGTTACCTGTTCATTTTTTATTAGATATTTTAAAGA
  CTTCAGAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001127328.1
• Summary: 'This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 34