ACCN1 (ASIC2) (NM_001094) Human 3' UTR Clone

CAT#: SC211215

3`UTR clone of amiloride-sensitive cation channel 1 neuronal (ACCN1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ASIC2
• Synonyms: ACCN; ACCN1; ASIC2a; BNaC1; BNC1; hBNaC1; MDEG
• ACCN: NM_001094
• Insert Size: 875 bp
• Sequence Data:
  Insert Sequence

  >SC211215 3'UTR clone of NM_001094
  The sequence shown below is from the reference sequence of NM_001094. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TTGGAGGAGATTGCCTGCTGACACCCCTCGAGTCACCCAGCACTCCCTCCAAACAGACCTTGAGGCCCAA
  GACCCAGGACAAGGAACAGCAAGCTCAGGTGGGATGGCCCCAGTGCTGGAAAGAAGCAAGAGCCCCCTAT
  GCACACATTGCAGACTAGCTGCCTAGACCTCGCTCCGGCCACGTCCAACACGACGCATCCTTGGGCCCCG
  CCGTGCGTCCCTCTTAGGAGAGATGAGTCACACTCTGGAACTGTCCAAGAACGAACCTGCCATCACATCT
  CACTGCCAGATGTATAAAGCACCTGCATGCTCAGACTTCTTGTGGCGCCACCTCCACGTCTGTCTTGTAC
  ATGACACTCCTCCACGCGGTTTCCAGTGTCCACACTGCTGCCCGTGCAGTGGGACCAGATTCCAGGTCCA
  AAGTCACCATGAGGCCACCCTGGAATCAGAACTGCACAATCAAGAGGGAACCCATGGGACTCTCTGCTAC
  ATTCAGTTCTTGTGTCGTTTGTGAAAGTTCTTAACCTGCCCAAAAACCCCCTTTTCCCCAAGCTGCCCAT
  GGGGCTTCGGCGCCAAAGGTGACCCGCGCCAACCTCCCTCCCCCCCAGTGCCTATGACGGCGGCACAGCA
  GCCAGCGGGTGGGGGACGCCTGTGTTCACCCATGGTGCCCATGTCGTTCTTCTCTCCCTGTGACACAGCT
  TGTACAGTCTGATTCTTTTTATCTGGGGTAGGGGGGCTTTTATGTTTGTCCGATGGAGATTTGTTTTGTT
  TTGCTTCATTTTATGCTTTTTTATTTTAGTTTTGATGTTCTGAGGTTTGCTTTGGTTTTTCCATTTTCTT
  TGGCATTTATTTATTCGTGCTTCAAATCACAGTCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001094.4
• Summary: 'This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified. [provided by RefSeq, Feb 2012]'
• Locus ID: 40