MEK1 (MAP2K1) (NM_002755) Human 3' UTR Clone

CAT#: SC211301

3`UTR clone of mitogen-activated protein kinase kinase 1 (MAP2K1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MAP2K1
• Synonyms: CFC3; MAPKK1; MEK1; MKK1; PRKMK1
• ACCN: NM_002755
• Insert Size: 923 bp
• Sequence Data:
  Insert Sequence

  >SC211301 3'UTR clone of NM_002755
  The sequence shown below is from the reference sequence of NM_002755. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CATGCTGCTGGCGTCTAAGTGTTTGGGAAGCAACAAAGAGCGAGTCCCCTGCCCGGTGGTTTGCCATGTC
  GCTTTTGGGCCTCCTTCCCATGCCTGTCTCTGTTCAGATGTGCATTTCACCTGTGACAAAGGATGAAGAA
  CACAGCATGTGCCAAGATTCTACTCTTGTCATTTTTAATATTACTGTCTTTATTCTTATTACTATTATTG
  TTCCCCTAAGTGGATTGGCTTTGTGCTTGGGGCTATTTGTGTGTATGCTGATGATCAAAACCTGTGCCAG
  GCTGAATTACAGTGAAATTTTGGTGAATGTGGGTAGTCATTCTTACAATTGCACTGCTGTTCCTGCTCCA
  TGACTGGCTGTCTGCCTGTATTTTCGGGATTCTTTGACATTTGGTGGTACTTTATTCTTGCTGGGCATAC
  TTTCTCTCTAGGAGGGAGCCTTGTGAGATCCTTCACAGGCAGTGCATGTGAAGCATGCTTTGCTGCTATG
  AAAATGAGCATCAGAGAGTGTACATCATGTTATTTTATTATTATTATTTGCTTTTCATGTAGAACTCAGC
  AGTTGACATCCAAATCTAGCCAGAGCCCTTCACTGCCATGATAGCTGGGGCTTCACCAGTCTGTCTACTG
  TGGTGATCTGTAGACTTCTGGTTGTATTTCTATATTTATTTTCAGTATACTGTGTGGGATACTTAGTGGT
  ATGTCTCTTTAAGTTTTGATTAATGTTTCTTAAATGGAATTATTTTGAATGTCACAAATTGATCAAGATA
  TTAAAATGTCGGATTTATCTTTCCCCATATCCAAGTACCAATGCTGTTGTAAACAACGTGTATAGTGCCT
  AAAATTGTATGAAAATCCTTTTAACCATTTTAACCTAGATGTTTAACAAATCTAATCTCTTATTCTAATA
  AATATACTATGAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002755.3
• Summary: 'The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]'
• Locus ID: 5604