Collagen XVII (COL17A1) (NM_000494) Human 3' UTR Clone

CAT#: SC211303

3`UTR clone of collagen type XVII alpha 1 (COL17A1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: COL17A1
• Synonyms: BA16H23.2; BP180; BPA-2; BPAG2; EBR2A; ERED; JEB-I; LAD-1
• ACCN: NM_000494
• Insert Size: 939 bp
• Sequence Data:
  Insert Sequence

  >SC211303 3'UTR clone of NM_000494
  The sequence shown below is from the reference sequence of NM_000494. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTATTGCTGTCAAGCCGTGAGCTAGCCATGGCAGGACAGCTCCTGGACCAGGTCTCATAATGCATGTGGC
  ACTTAGGTCCAAGGTCTCCAGAGGGTGAAAGCTGGAGTCTGTCAATGTCCTACTGAGACAGCACAGCCAA
  CCTAGCTAGCAACATTTGTTTTAGTCTGAACAATATATACTTATAGAATTCAGTCAAAGATACACAATCT
  GAAACAGCTTCATGGGGTGGACTCTAACAGTAGTTGCAATGTTTTAGAATGAGACTTACTTCTCTGCTAT
  CTAGATCTGAACTCCTTGGCTTCTTTACTTAGTTCAAGCCCCAGCCTAGGAAAGCCAGTTACATAAAAGT
  TGGCTCAGGAGTCTTAGAGCTTTACCTAAATATGAGCCCAGAAAACGGAGGATGGGGGTGGGGCGCCTTC
  CTGGAGGTGACACTTGATGGGGGTGTGTTCTGGTTACTGTTCTAAGGCTGTGCCATCAGCTCCTTCCTCC
  CCTGTTCATTCTGCATTCTCTAGTCAGTTGGCTAAGAAGTGACTCTTGCAACTAAAAAAATTAAGAAATT
  CACTTCCCCTCTAGGAGGTGATGATAGGGTTTCTAATGGTTATATGTATATCACATTCCCATTTGCTTAG
  AAAGTCTGATTGTAGCTATGATTGTCCGTAGGCCCATACTAGAGTTCATGGATATGTTATACTGAACCAG
  GCCAGAGCAAACAGAAAAAGAAGGTTGAGGGCAATGGACAAGGAAGGAATAAAGGGAGAAGAGGGAAAAC
  AGAAAACCTGATGCTGGGGACACAGCATCAGCTCAAGACGTCACCCTCCATTCTGCACTCAGAAAATGGC
  ACTTGGGGGACTGGGCGCAGTTGGTCTTTAACCACTTTTCAATGTCTAAAAACATTTGTTTGTGGTCTAT
  AAGATGAAACATCATTTCAATCGTAAAAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000494.3
• Summary: 'This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008]'
• Locus ID: 1308