uPA (PLAU) (NM_002658) Human 3' UTR Clone

CAT#: SC211329

3`UTR clone of plasminogen activator urokinase (PLAU) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PLAU
• Synonyms: ATF; BDPLT5; QPD; u-PA; UPA; URK
• ACCN: NM_002658
• Insert Size: 962 bp
• Sequence Data:
  Insert Sequence

  >SC211329 3'UTR clone of NM_002658
  The sequence shown below is from the reference sequence of NM_002658. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CACCAAGGAAGAGAATGGCCTGGCCCTCTGAGGGTCCCCAGGGAGGAAACGGGCACCACCCGCTTTCTTG
  CTGGTTGTCATTTTTGCAGTAGAGTCATCTCCATCAGCTGTAAGAAGAGACTGGGAAGATAGGCTCTGCA
  CAGATGGATTTGCCTGTGCCACCCACCAGGGCGAACGACAATAGCTTTACCCTCAGGCATAGGCCTGGGT
  GCTGGCTGCCCAGACCCCTCTGGCCAGGATGGAGGGGTGGTCCTGACTCAACATGTTACTGACCAGCAAC
  TTGTCTTTTTCTGGACTGAAGCCTGCAGGAGTTAAAAAGGGCAGGGCATCTCCTGTGCATGGGTGAAGGG
  AGAGCCAGCTCCCCCGACGGTGGGCATTTGTGAGGCCCATGGTTGAGAAATGAATAATTTCCCAATTAGG
  AAGTGTAACAGCTGAGGTCTCTTGAGGGAGCTTAGCCAATGTGGGAGCAGCGGTTTGGGGAGCAGAGACA
  CTAACGACTTCAGGGCAGGGCTCTGATATTCCATGAATGTATCAGGAAATATATATGTGTGTGTATGTTT
  GCACACTTGTGTGTGGGCTGTGAGTGTAAGTGTGAGTAAGAGCTGGTGTCTGATTGTTAAGTCTAAATAT
  TTCCTTAAACTGTGTGGACTGTGATGCCACACAGAGTGGTCTTTCTGGAGAGGTTATAGGTCACTCCTGG
  GGCCTCTTGGGTCCCCCACGTGACAGTGCCTGGGAATGTATTATTCTGCAGCATGACCTGTGACCAGCAC
  TGTCTCAGTTTCACTTTCACATAGATGTCCCTTTCTTGGCCAGTTATCCCTTCCTTTTAGCCTAGTTCAT
  CCAATCCTCACTGGGTGGGGTGAGGACCACTCCTGTACACTGAATATTTATATTTCACTATTTTTATTTA
  TATTTTTGTAATTTTAAATAAAAGTGATCAATAAAATGTGATTTTTCTGATG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002658.3
• Summary: 'This gene encodes a secreted serine protease that converts plasminogen to plasmin. The encoded preproprotein is proteolytically processed to generate A and B polypeptide chains. These chains associate via a single disulfide bond to form the catalytically inactive high molecular weight urokinase-type plasminogen activator (HMW-uPA). HMW-uPA can be further processed into the catalytically active low molecular weight urokinase-type plasminogen activator (LMW-uPA). This low molecular weight form does not bind to the urokinase-type plasminogen activator receptor. Mutations in this gene may be associated with Quebec platelet disorder and late-onset Alzheimer's disease. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]'
• Locus ID: 5328